rat primary cortical astrocytes Search Results


rat primary cortical astrocytes  (Thermo Fisher)
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Gibco Rat Primary Cortical Astrocytes are isolated and cryopreserved from the cortex of Sprague Dawley Rats at day E19 of gestation. A flexible, ready-to-use, and quality alternative to fresh astrocytes, each vial contains 1 million
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primary rat cortical astrocytes  (Japan SLC inc)
90
Japan SLC inc primary rat cortical astrocytes
Selective activation of the engineered M1R in primary rat cortical <t>astrocytes.</t> (a) Schematic illustration of selective activation of the His 4 tag-fused M1R over endogenous mAChRs by coordination tethering. (b) Confocal microscopic images of astrocytes co-transfected with AcGFP (green) and M1R(1.18H4) immunostained by an anti-HA tag antibody (magenta). Nuclei were stained with Hoechst33258 (blue). Scale bar: 20 μm. Ca 2+ responses evoked by 30 nM (c) MAC(M1R) and (d) control-2 in astrocytes harboring the plasmids of (red) M1R(1.18H4), (blue) WT M1R, or (black) the control vector ( n = 18). Fura-2 ratiometric images of astrocytes expressing M1R(1.18H4) (e) before and (f) during perfusion of 30 nM MAC(M1R) . Red and blue represent high and low Ca 2+ concentration, respectively. Scale bar: 100 μm. (g) Average Δratio plot evoked by 5 min of treatment of 30 nM MAC(M1R) and control-2 ( n = 11–23). *** denote significant differences from the group of M1R(1.18H4)/ MAC(M1R) /inhibitor(−) (***: P < 0.001, one way analysis of variance with Dunnett’s post hoc test). F(6/117) = 23.05. Concentration–response curves of (h) M1R(1.18H4) and (i) WT M1R on (red) MAC(M1R) and (black) control-2 ( n = 20–25). The data represent the mean ± SEM. Reproducibility of all experiments was confirmed at least two times.
Primary Rat Cortical Astrocytes, supplied by Japan SLC inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary rat cortical astrocytes - by Bioz Stars, 2026-09
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Image Search Results


Selective activation of the engineered M1R in primary rat cortical astrocytes. (a) Schematic illustration of selective activation of the His 4 tag-fused M1R over endogenous mAChRs by coordination tethering. (b) Confocal microscopic images of astrocytes co-transfected with AcGFP (green) and M1R(1.18H4) immunostained by an anti-HA tag antibody (magenta). Nuclei were stained with Hoechst33258 (blue). Scale bar: 20 μm. Ca 2+ responses evoked by 30 nM (c) MAC(M1R) and (d) control-2 in astrocytes harboring the plasmids of (red) M1R(1.18H4), (blue) WT M1R, or (black) the control vector ( n = 18). Fura-2 ratiometric images of astrocytes expressing M1R(1.18H4) (e) before and (f) during perfusion of 30 nM MAC(M1R) . Red and blue represent high and low Ca 2+ concentration, respectively. Scale bar: 100 μm. (g) Average Δratio plot evoked by 5 min of treatment of 30 nM MAC(M1R) and control-2 ( n = 11–23). *** denote significant differences from the group of M1R(1.18H4)/ MAC(M1R) /inhibitor(−) (***: P < 0.001, one way analysis of variance with Dunnett’s post hoc test). F(6/117) = 23.05. Concentration–response curves of (h) M1R(1.18H4) and (i) WT M1R on (red) MAC(M1R) and (black) control-2 ( n = 20–25). The data represent the mean ± SEM. Reproducibility of all experiments was confirmed at least two times.

Journal: ACS Central Science

Article Title: Chemogenetic Approach Using Ni(II) Complex–Agonist Conjugates Allows Selective Activation of Class A G-Protein-Coupled Receptors

doi: 10.1021/acscentsci.8b00390

Figure Lengend Snippet: Selective activation of the engineered M1R in primary rat cortical astrocytes. (a) Schematic illustration of selective activation of the His 4 tag-fused M1R over endogenous mAChRs by coordination tethering. (b) Confocal microscopic images of astrocytes co-transfected with AcGFP (green) and M1R(1.18H4) immunostained by an anti-HA tag antibody (magenta). Nuclei were stained with Hoechst33258 (blue). Scale bar: 20 μm. Ca 2+ responses evoked by 30 nM (c) MAC(M1R) and (d) control-2 in astrocytes harboring the plasmids of (red) M1R(1.18H4), (blue) WT M1R, or (black) the control vector ( n = 18). Fura-2 ratiometric images of astrocytes expressing M1R(1.18H4) (e) before and (f) during perfusion of 30 nM MAC(M1R) . Red and blue represent high and low Ca 2+ concentration, respectively. Scale bar: 100 μm. (g) Average Δratio plot evoked by 5 min of treatment of 30 nM MAC(M1R) and control-2 ( n = 11–23). *** denote significant differences from the group of M1R(1.18H4)/ MAC(M1R) /inhibitor(−) (***: P < 0.001, one way analysis of variance with Dunnett’s post hoc test). F(6/117) = 23.05. Concentration–response curves of (h) M1R(1.18H4) and (i) WT M1R on (red) MAC(M1R) and (black) control-2 ( n = 20–25). The data represent the mean ± SEM. Reproducibility of all experiments was confirmed at least two times.

Article Snippet: Primary rat cortical astrocytes were obtained from P2 neonatal SD rat pups (both male and female were used) (Japan SLC) according to the protocol reported by Guaza et al .

Techniques: Activation Assay, Transfection, Staining, Control, Plasmid Preparation, Expressing, Concentration Assay